Stepwise Development of Hematopoietic Stem Cells from Embryonic Stem Cells
نویسندگان
چکیده
منابع مشابه
Stepwise Development of Hematopoietic Stem Cells from Embryonic Stem Cells
The cellular ontogeny of hematopoietic stem cells (HSCs) remains poorly understood because their isolation from and their identification in early developing small embryos are difficult. We attempted to dissect early developmental stages of HSCs using an in vitro mouse embryonic stem cell (ESC) differentiation system combined with inducible HOXB4 expression. Here we report the identification of ...
متن کاملDifferentiation of Mouse Embryonic Stem Cells into Hematopoietic Cells
Purpose: Differentiation of Mouse embryonicstem cells into Hematopoietic cells. Materials and Methods: In this study, we used EB formation system for Hematopoietic differentiation of mouse embryonic stem cell (Royan B1) in suspension culture. EBs cultured in medium with Hematopoietic inducer cytokines (SCF, TPO, GMCSF, IL3, Flt3 and EPO) .presence of hematopoietic differentiated cell assessed ...
متن کاملDevelopment of hematopoietic repopulating cells from embryonic stem cells.
A reliable and effective in vitro differentiation system is described for the production of functional neutrophils from mouse embryonic stem (ES) cells. A three-step culture method was developed that enables abundant production and effective harvesting of mature neutrophils at high purity without sorting. Utilization of the OP9 stromal cell line, which does not produce macrophage colony stimula...
متن کاملDerivation of hematopoietic stem cells from murine embryonic stem cells.
A stem cell is defined as a cell with the capacity to both self-renew and generate multiple differentiated progeny. Embryonic stem cells (ESC) are derived from the blastocyst of the early embryo and are pluripotent in differentiative ability. Their vast differentiative potential has made them the focus of much research centered on deducing how to coax them to generate clinically useful cell typ...
متن کاملEmbryonic stem cell-derived hematopoietic stem cells.
Despite two decades of studies documenting the in vitro blood-forming potential of murine embryonic stem cells (ESCs), achieving stable long-term blood engraftment of ESC-derived hematopoietic stem cells in irradiated mice has proven difficult. We have exploited the Cdx-Hox pathway, a genetic program important for blood development, to enhance the differentiation of ESCs along the hematopoietic...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: PLoS ONE
سال: 2009
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0004820